There are over 750 human enzymes dedicated to glycan synthesis, catabolism and recognition. They include glycosyltrasferases (GTs) and glycoside hydrolases (GHs). While there is tremendous demand for these enzymes in the scientific community, few are available in sufficient quantities for synthetic purposes or for structural/functional studies. Not surprisingly, glyco-enzymes are exceptionally underrepresented in the Protein Data Bank.
To help overcome these bottlenecks, NIGMS is partnering with NIH’s National Center for Research Resources to provide a two-year Recovery Act supplement to the NCRR-sponsored Resource for Integrated Glycotechnology at the University of Georgia. The center will draw additional expertise from investigators at the University of Arizona and University of Wyoming to generate libraries of gateway and expression vectors for glyco-enzymes. The gateway and expression libraries for these enzymes will begin to be made available to the scientific community over the next few months.
The team also will work to express and distribute a subset of these enzymes. Your input for this expression effort is welcome. Please direct inquiries regarding these vectors/enzymes to Kelley Moremen.
This new repository for mammalian GT and GH libraries will speed expansion of the chemical space for carbohydrates as well as speed structural and biochemical studies of these enzymes. The resource should benefit multiple scientific communities and accelerate progress on both the basic biology of the enzymes and their use for development of screening tools (arrays), diagnostics and therapeutics.
The GT and GH expression vectors libraries also may be a useful resource for researchers planning to respond to the upcoming PSI:Biology program announcements mentioned in an earlier post.