Expression Plasmids and Empty Vectors Available

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PSI:Biology-Materials Repository bannerGreat news for biochemists, biologists and structural biologists—more than 50,000 protein expression plasmids and almost 100 empty vectors are now available through the PSI:Biology-Materials Repository Link to external web site. This includes about 900 membrane protein plasmids, and we expect this number—plus that for human proteins—to grow in the coming months.

The repository has carefully collected, maintained and annotated these materials generated by scientists involved in the Protein Structure Initiative. In addition, it has developed and optimized the empty vectors for producing proteins in bacteria, yeast and cell-free systems. For a modest charge, you can order the plasmids and vectors from the online catalog Link to external web site.

Many of the plasmids represent proteins whose crystal structures have been determined but whose biological functions are not yet known. Search the repository Link to external web site or use the Functional Sleuth  Link to external web site to find out if the structure of your favorite protein or a similar one has already been determined.

If you can’t locate the plasmids you need in the PSI collection, you might search the larger DNASU plasmid repository Link to external web site, which houses the PSI:Biology Materials Repository. This central repository offers plasmids from hundreds of organisms and special collections, including human kinases, the Thermotoga maritime genome and a new set of 180 glycoenzymes.

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